INVESTIGADORES
BIGLIONE Mirna Marcela
artículos
Título:
HDAg-L VARIANTS IN COVERT HEPATITIS D AND HBV OCCULT INFECTION AMONG
Autor/es:
DELFINO C; EIRIN ME; BERII C; MALAN R; GENTILE E; CASTILLO A; PEDROZO W; KRUPP R; BLEJER J; OUBIÑA J; MATHET V; BIGLIONE MM
Revista:
JOURNAL OF CLINICAL VIROLOGY : THE OFFICIAL PUBLICATION OF THE PAN AMERICAN SOCIETY FOR CLINICAL VIROLOGY.
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Londrés; Año: 2012 vol. 54 p. 223 - 228
ISSN:
1386-6532
Resumen:
Background: Guidelines suggest that all HBsAg-positive patients should be tested for anti-HDV IgG antibodies
and to confirm active hepatitis D virus (HDV) infection by detection of HDV RNA by reverse
transcriptase (RT) polymerase chain reaction (PCR).
and to confirm active hepatitis D virus (HDV) infection by detection of HDV RNA by reverse
transcriptase (RT) polymerase chain reaction (PCR).
and to confirm active hepatitis D virus (HDV) infection by detection of HDV RNA by reverse
transcriptase (RT) polymerase chain reaction (PCR).
and to confirm active hepatitis D virus (HDV) infection by detection of HDV RNA by reverse
transcriptase (RT) polymerase chain reaction (PCR).
and to confirm active hepatitis D virus (HDV) infection by detection of HDV RNA by reverse
transcriptase (RT) polymerase chain reaction (PCR).
Guidelines suggest that all HBsAg-positive patients should be tested for anti-HDV IgG antibodies
and to confirm active hepatitis D virus (HDV) infection by detection of HDV RNA by reverse
transcriptase (RT) polymerase chain reaction (PCR).
Objectives: The aim of this study was to determine the serological prevalence and molecular features of
HDV within an Amerindian community from Argentina exhibiting positivity for HBsAg and/or anti-HBc
total Ig.
HDV within an Amerindian community from Argentina exhibiting positivity for HBsAg and/or anti-HBc
total Ig.
HDV within an Amerindian community from Argentina exhibiting positivity for HBsAg and/or anti-HBc
total Ig.
HDV within an Amerindian community from Argentina exhibiting positivity for HBsAg and/or anti-HBc
total Ig.
HDV within an Amerindian community from Argentina exhibiting positivity for HBsAg and/or anti-HBc
total Ig.
The aim of this study was to determine the serological prevalence and molecular features of
HDV within an Amerindian community from Argentina exhibiting positivity for HBsAg and/or anti-HBc
total Ig.
Study design: Forty-six plasma samples were tested for the detection of total anti-HDV antibodies by
ELISA. Concomitantly, a partial RNA region coding for the delta antigen (HDAg) was amplified by RTnested
PCR (RT-nPCR). In silica translation of DNA sequences into the amino acid (aa) sequence of HDAg-S
(aa110-198) and HDAg-L (AA110-214) was performed.
ELISA. Concomitantly, a partial RNA region coding for the delta antigen (HDAg) was amplified by RTnested
PCR (RT-nPCR). In silica translation of DNA sequences into the amino acid (aa) sequence of HDAg-S
(aa110-198) and HDAg-L (AA110-214) was performed.
ELISA. Concomitantly, a partial RNA region coding for the delta antigen (HDAg) was amplified by RTnested
PCR (RT-nPCR). In silica translation of DNA sequences into the amino acid (aa) sequence of HDAg-S
(aa110-198) and HDAg-L (AA110-214) was performed.
ELISA. Concomitantly, a partial RNA region coding for the delta antigen (HDAg) was amplified by RTnested
PCR (RT-nPCR). In silica translation of DNA sequences into the amino acid (aa) sequence of HDAg-S
(aa110-198) and HDAg-L (AA110-214) was performed.
ELISA. Concomitantly, a partial RNA region coding for the delta antigen (HDAg) was amplified by RTnested
PCR (RT-nPCR). In silica translation of DNA sequences into the amino acid (aa) sequence of HDAg-S
(aa110-198) and HDAg-L (AA110-214) was performed.
Forty-six plasma samples were tested for the detection of total anti-HDV antibodies by
ELISA. Concomitantly, a partial RNA region coding for the delta antigen (HDAg) was amplified by RTnested
PCR (RT-nPCR). In silica translation of DNA sequences into the amino acid (aa) sequence of HDAg-S
(aa110-198) and HDAg-L (AA110-214) was performed.and HDAg-L (AA110-214) was performed.
Results: Out of 46 HDV non-reactive samples by ELISA, 3 were HDV RNA positive by RT-nPCR. These
samples were anti-HBc-only positive, 2 of them identified as cases of occult hepatitis B infection (OBI).
The 3 cases were HBeAg-negative and showed normal ALT/AST levels. All sequences were ascribed to
HDV genotype 1, but exhibited nucleotide differences in HDAg-L coding region, among which, mutations
at codons 197 and 201 -reportedly known to promote in vitro an unsuitable interaction with HBsAg-
samples were anti-HBc-only positive, 2 of them identified as cases of occult hepatitis B infection (OBI).
The 3 cases were HBeAg-negative and showed normal ALT/AST levels. All sequences were ascribed to
HDV genotype 1, but exhibited nucleotide differences in HDAg-L coding region, among which, mutations
at codons 197 and 201 -reportedly known to promote in vitro an unsuitable interaction with HBsAg-
samples were anti-HBc-only positive, 2 of them identified as cases of occult hepatitis B infection (OBI).
The 3 cases were HBeAg-negative and showed normal ALT/AST levels. All sequences were ascribed to
HDV genotype 1, but exhibited nucleotide differences in HDAg-L coding region, among which, mutations
at codons 197 and 201 -reportedly known to promote in vitro an unsuitable interaction with HBsAg-
samples were anti-HBc-only positive, 2 of them identified as cases of occult hepatitis B infection (OBI).
The 3 cases were HBeAg-negative and showed normal ALT/AST levels. All sequences were ascribed to
HDV genotype 1, but exhibited nucleotide differences in HDAg-L coding region, among which, mutations
at codons 197 and 201 -reportedly known to promote in vitro an unsuitable interaction with HBsAg-
samples were anti-HBc-only positive, 2 of them identified as cases of occult hepatitis B infection (OBI).
The 3 cases were HBeAg-negative and showed normal ALT/AST levels. All sequences were ascribed to
HDV genotype 1, but exhibited nucleotide differences in HDAg-L coding region, among which, mutations
at codons 197 and 201 -reportedly known to promote in vitro an unsuitable interaction with HBsAg-
Out of 46 HDV non-reactive samples by ELISA, 3 were HDV RNA positive by RT-nPCR. These
samples were anti-HBc-only positive, 2 of them identified as cases of occult hepatitis B infection (OBI).
The 3 cases were HBeAg-negative and showed normal ALT/AST levels. All sequences were ascribed to
HDV genotype 1, but exhibited nucleotide differences in HDAg-L coding region, among which, mutations
at codons 197 and 201 -reportedly known to promote in vitro an unsuitable interaction with HBsAg-known to promote in vitro an unsuitable interaction with HBsAg-
were observed.
Conclusions: These results provide evidence of covert HDV infection even among OBI, highlighting the
need to reevaluate the currently applied guidelines for HDV diagnostic algorithms, as well as to explore
if the observed mutations promote any effect on HDV pathogenesis.
need to reevaluate the currently applied guidelines for HDV diagnostic algorithms, as well as to explore
if the observed mutations promote any effect on HDV pathogenesis.
need to reevaluate the currently applied guidelines for HDV diagnostic algorithms, as well as to explore
if the observed mutations promote any effect on HDV pathogenesis.
need to reevaluate the currently applied guidelines for HDV diagnostic algorithms, as well as to explore
if the observed mutations promote any effect on HDV pathogenesis.
need to reevaluate the currently applied guidelines for HDV diagnostic algorithms, as well as to explore
if the observed mutations promote any effect on HDV pathogenesis.
These results provide evidence of covert HDV infection even among OBI, highlighting the
need to reevaluate the currently applied guidelines for HDV diagnostic algorithms, as well as to explore
if the observed mutations promote any effect on HDV pathogenesis.