INVESTIGADORES
CURUTCHET Gustavo Andres
artículos
Título:
PDMS based porous particles as support beds for cell immobilization:bacterial biofilm formation as a function of porosity and polymer composition
Autor/es:
M. FERNANDEZ; G. CASABONA; V. ANUPAMA; G. CURUTCHET; D. BERNIK
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2010 vol. 81 p. 289 - 296
ISSN:
0927-7765
Resumen:
The objective of thisworkis to test the performance ofnewsynthetic polydimethylsiloxane (PDMS)-based
bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected
porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials
can be cross-linked by copolymerization with other polymers. In the present work we have chosen
two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile
composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface
level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy.
Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing BacteriaSulphate Reducing Bacteria
(SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles
synthesized with pure PDMS, PDMSxanthan gum and PDMSTEOS hybrids. Bacterial viability assays
using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on
particles surface significantly improves both cell adhesion and viability
synthesized with pure PDMS, PDMSxanthan gum and PDMSTEOS hybrids. Bacterial viability assays
using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on
particles surface significantly improves both cell adhesion and viability
SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles
synthesized with pure PDMS, PDMSxanthan gum and PDMSTEOS hybrids. Bacterial viability assays
using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on
particles surface significantly improves both cell adhesion and viability