Ploidy of south american catfish Rhamdia sapo (Pisces, Pimelodidae) determined by spectrofluorometry

SCHWARZBAUM PJ, VALCARCEL A, MAGGESE MC

JOURNAL OF AQUACULTURE IN THE TROPICS

Swets & Zeitlinger Publishers

Año: 1992 p. 151 - 155

0970-0846

a method is described to estimate ploidy in erythrocyte suspension by fluorescence spectrophotometry using a DNA intercalating fluorescent dye. Fluorescence spectrophotometry provided the identification of diploid and polyploid individuals. The DNA fluorescence of South American catfish (Rhamdia sapo) erythrocytes was 15.59% that of the chicken reference. The advantage of this technique is discussed herein