INVESTIGADORES
FERRERO Mariana Cristina
artículos
Título:
Direct and monocyte-induced innate immune response of human lung
Autor/es:
MARIANA C FERRERO; CARLOS A FOSSATI; PABLO C BALDI
Revista:
MICROBES AND INFECTION
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2010 vol. 12 p. 736 - 747
ISSN:
1286-4579
Resumen:
Although Brucella frequently infects humans through inhalation, its interaction with pulmonary cells has been overlooked. We examined
whether human lung epithelial cells produce proinflammatory mediators in response to Brucella infection. Infection with smooth or rough strains
of Brucella abortus induced the secretion of IL-8 and GM-CSF by the bronchial epithelial cell lines Calu-6 and 16HBE14o-, but not by the
alveolar epithelial cell line A549. Infected Calu-6 cells also produced low levels of MCP-1. Since monocyte-derived cytokines may induce
chemokine secretion in epithelial cells, cocultures of human monocytes (THP-1 cell line) and respiratory epithelial cells were used to study such
interaction. IL-8 and MCP-1 levels in B. abortus-infected THP-1:A549 and THP-1:Calu-6 cocultures, and MCP-1 levels in THP-1:16HBE14ococultures, were higher than those detected in infected epithelial monocultures. Conditioned medium from infected monocytes induced the
secretion of IL-8 and/or MCP-1 by A549 and Calu-6 cells, and these effects were mainly mediated by IL-1 (in A549 cells) or TNF-a (in Calu-6
cells). Conversely, culture supernatants from Brucella-infected bronchial epithelial cells induced MCP-1 production by monocytes, an effect
largely mediated by GM-CSF. This study shows that human lung epithelial cells mount a proinflammatory response to Brucella, either directly or
after interaction with Brucella-infected monocytes