Compared Lectin binding pattern in the cerebellar cortical degeneration I nduced by Solanum fastigiatum var. fastigiatum and Solanum bonariense in cattle.

SANT`ANA F; BARBEITO CG; NISHIDA F; GIMENO EJ; VERDES JM,; BATTES D; MORAÑA A; BARROS C

International Journal of Poisonous Plant Research

USDA-ARS Poisonous Plant Research Laboratory

Lugar: Logan; Año: 2011 vol. 1 p. 28 - 34

2154-3216

Microscopic and lectin histochemical studies were performed using the cerebella of 33natural cases of Solanum fastigiatum var. fastigiatum intoxication in cattle from southernBrazil and 2 natural and 4 experimental cases of Solanum bonariense from Uruguay. The following biotinylated lectins were used in both cases: WGA, sWGA, BS-I, Con-A,RCA-I, DBA, and UEA-I, with the addition of LCA in S. fastigiatum poisoning cases.Histologically, the lesions consisted of fine vacuolization, distention of portions of the Purkinje cells, axonal spheroids measuring 14-50 μm in the granular cell layer andadjacent white matter and, proliferation of the Bergmann’s glia. Lectin histochemistry revealed strong reactivity of stored material in Purkinje neurons with the lectins sWGA,Con-A, and LCA in S. fastigiatum cases. A similar pattern was found in S. bonariensecases with a most intense reaction to WGA, and less intense reaction to Con-A, whereasBS-I and RCA-I binding was absent to poor in these neurons in all the cases studied. Lectin reactivity in Purkinje cells between cases was independent of cell damage (from mild to severe loss of neurons). Both S. fastigiatum and S. bonariense have similar lectin binding, suggesting a similar pathogenesis. Since comparable binding patterns have beendescribed in animals poisoned with swainsonine-containing plants, perhaps the toxins inthese plants contain related glycosidase-inhibiting toxins or inhibit glycoprotein andlysosomal metabolism through some related mechanism. The results of this study showedthat in spontaneous poisoning by S. fastigiatum and S. bonariense in cattle, the pattern of lectin binding is similar to those observed in S. fastigiatum experimental conditions.