Improved in vitro oocyte maturation and IVF efficiency in pigs by supplementation with metformin and insulin-transferrin-selenium (ITS).

LUCHETTI, CG.; LORENZO, MS.; ELIA, E.; TEPLITZ, G.; CRUZANS, PR.; LOMBARDO, DM.

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2022

1031-3613

Oxidative stress and polyspermy are among the main causes of the low efficiency of in vitro fertilization (IVF) in pigs. Oxidative stress is triggered by the in vitro conditions and is reflected in long-term consequences on implantation and fetal development. Polyspermy is probably a product of an incorrect cortical reaction in this species and has short-term consequences on the formation of the zygote.The aim of this work was to assess the effects of supplementation of porcine invitro maturation (IVM) media with the antioxidants metformin (M) and insulin28 transferrin-selenium (ITS). Cumulus-oocyte complexes (COC) were subjected to IVM for 44-46 h at 39°C, 5% CO2 and humidified chamber in supplemented TCM30 199. Experimental groups: control, M (10-4M), ITS (0.1% v/v) and M+ITS. The nuclear maturation rate increased with M+ITS (P< 0.05). Glucose consumption increased with ITS and M+ITS (P< 0.005). Redox balance (in homogenized COC and 96-well plates): glutathione increased and lipid peroxidation decreased with M, ITS and with M+ITS (P< 0.005), protein concentration and superoxide dismutase did not show differences. The viability of cumulus cells (flow cytometry) increased with M and decreased with ITS (P< 0.005). After IVF, presumptive zygotes showed penetration increased with M, male pronucleus formation decreased with M, and IVF efficiency increased with M+ITS (P< 0.05).These results suggest that the supplementation with M+ITS improves IVM and IVF in pigs, increasing nuclear maturation and glucose consumption, decreasing oxidative stress and increasing IVF efficiency.