Extraction of biogenic amines and their dansyl derivatives with reverse microemulsions of bis [2-ethylhexyl] sulphosuccinate (AOT) prior to high-performance liquid chromatographic determination

L. ROMERO, S. KEUNCHKARIAN, M. RETA

ANALYTICA CHIMICA ACTA

Elsevier

Año: 2006 vol. 565 p. 136 - 144

0003-2670

The ability of reverse microemulsions of the surfactant bis [2-ethylhexyl] sulphosuccinate (AOT) in heptane to extract and preconcentrate eightbiogenic amines present in aqueous matrices has been explored. The “phase transfer” method, in which the surfactant solution is contacted withan aqueous salt solution containing the analytes, has been used as the analytical methodology. The extraction efficiencies have been comparedwith those obtained by using the “cloud point” extraction technique with Triton X-114 as surfactant, and with methylene chloride as a typicalextracting solvent. In order to improve extraction and detection as compared with the underivatized amines, the fluorescent reagent dansyl chloridehas been used to obtain the more hydrophobic and thus, easily extractable dansyl derivatives. Although extraction of most of the biogenic amines ishigh, extraction of their dansyl derivatives is practically 100%. The procedure was successfully applied to the analysis of commercial fruit juices.The analytical methodology is sensitive, fast, simple and reproducible. The LOD were in the vicinity of 1 and 2 pmol, which is similar or betterthan other techniques. The micellar phase is compatible with reverse phase HPLC mobile phases, which allow direct injection of the analyte afterextraction. This is an advantage over solid phase extraction (SPE), where incomplete recovery can occur. Moreover, AOT showed to be betterextracting surfactant for biogenic amines (and their corresponding dansyl derivatives) than Triton X-114 at the same concentration.