INVESTIGADORES
FERREIRO Diego Ulises
artículos
Título:
Experimental snapshots of a protein-DNA binding landscape.
Autor/es:
SÁNCHEZ IE *, FERREIRO DU *, DELLAROLE M, DE PRAT-GAY G.
Revista:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Editorial:
NATL ACAD SCIENCES
Referencias:
Año: 2010 vol. 107 p. 7751 - 7756
ISSN:
0027-8424
Resumen:
Protein recognition of DNA sites is a primary event for gene function.
Its ultimate mechanistic understanding requires an integrated
structural, dynamic, kinetic, and thermodynamic dissection that is
currently limited considering the hundreds of structures of protein-DNA
complexes available. We describe a protein-DNA-binding pathway in which
an initial, diffuse, transition state ensemble with some nonnative
contacts is followed by formation of extensive nonnative interactions
that drive the system into a kinetic trap. Finally, nonnative contacts
are slowly rearranged into native-like interactions with the DNA
backbone. Dissimilar protein-DNA interfaces that populate along the
DNA-binding route are explained by a temporary degeneracy of protein-DNA
interactions, centered on "dual-role" residues. The nonnative species
slow down the reaction allowing for extended functionality.