INVESTIGADORES
FERREIRO Diego Ulises
artículos
Título:
Consequences of localized frustration for the folding mechanism of the IM7 protein.
Autor/es:
SUTTO L, LÄTZER J, HEGLER JA, FERREIRO DU, WOLYNES PG.
Revista:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Referencias:
Año: 2007 vol. 104 p. 19825 - 19830
ISSN:
0027-8424
Resumen:
In the laboratory, IM7 has been found to have an
unusual folding mechanism in which an "on-pathway" intermediate with
nonnative interactions is formed. We show that this intermediate is a
consequence of an unusual cluster of highly frustrated interactions in
the native structure. This cluster is involved in the binding of IM7 to
its target, Colicin E7. Redesign of residues in this cluster to
eliminate frustration is predicted by simulations to lead to faster
folding without the population of an intermediate ensemble.