DIETARY SUPPLEMENTATION WITH INTEGRAL CHIA AND FLAX FLOURS AMELIORATES SYSTEMIC INFLAMMATION

COLUSSI, N.A.; TODARO SANTIAGO; RODRIGUEZ JUAN PABLO; GABRIELA OLEA; FERRINI LEANDRO; STOYANOFF TANIA; AGUIRRE MA. VICTORIA

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2024 vol. 84 p. 206 - 220

0025-7680

Introduction: Chia and flax seeds are rich in alphalinolenic acid (ALA), which is bioconverted into the active derivatives eicosapentaenoic (EPA) and docosahexaenoic (DHA) having multiple beneficial effects. However, there is limited knowledge about the antiinflammatory effects of chia and flax integral flours diets rich in ALA. Objective: The study aimed to evaluate the ntiinflammatory effect of dietary supplementation with integral chia and flax flours in a murine model of LPSinduced systemic inflammation. Methods: Balb/c mice were distributed into threegroups: diet A (control), diet B (supplemented with integral chia flour), and diet C (supplemented with integral flax flour). Nutritional, hematological, and biochemical determinations were performed. ALA, EPA, and DHA were assessed by GC-MS in the liver, brain, cardiacand skeletal muscles. NF-kB immunoassays were performed in kidney, liver, and peritoneal macrophages, respectively. The phagocytic capacity was determined in peritoneal macrophages and the expression of the pro- and anti-inflammatory cytokines was assessed byRT-qPCR in the kidney, liver, and spleen. Results: Diets B and C exhibited optimal nutritionaladequacy and caused increased levels of ALA, EPA, and DHA in critical tissues compared to the control. The phagocytic capacity of murine peritoneal macrophages (p< 0.01) and IL-10 transcription increased, whereas the expression of NF-κB, IL-1β, IL-6, and TNF-α decreased inanimals fed both experimental diets. Conclusions: This work contributes to the currentknowledge of the anti-inflammatory effects of chia and flax integral flours rich in ALA and reinforces the health advantages of their consumption.