INVESTIGADORES
DEL POPOLO Mario Gabriel
artículos
Título:
Birnaviral Hijacking of Endosomal Membranes
Autor/es:
FLAVIA A. ZANETTI; IGNACIO FERNANDEZ; EDUARD BAQUERO; PABLO GUARDADO-CALVO; SARAH DUBOIS; ETIENNE MOREL; VICTORIA ALFONSO; MILTON O. AGUILERA; MARÍA E. CELAYES; LUIS M. POLO; LAILA SUHAIMAN; M. VANINA CHIARPOTTI; CAROLINA ALLENDE; JAVIER M. RODRIGUEZ; JOSÉ R. CASTÓN; DIEGO LIJAVETZKY; OSCAR TABOGA; MARÍA I. COLOMBO; MARIO G. DEL PÓPOLO; FÉLIX S. REY; LAURA R. DELGUI
Revista:
eLife
Editorial:
eLife Sciences Publications Ltd.
Referencias:
Lugar: Cambridge; Año: 2024
ISSN:
2050-084X
Resumen:
Birnaviruses form a distinct class of double-stranded RNA (dsRNA) viruses characterized by the absence of a transcription-competent inner core particle. The early endosomes (EE) of cells infected with the infectious bursal disease virus (IBDV) - a prototypical birnavirus and an important avian pathogen - constitute a platform for viral replication. Here, we study the mechanism of birnaviral hijacking of EE membranes for this process. We demonstrate that the viral protein 3 (VP3) specifically binds to phosphatidylinositol-3-phosphate (PI3P) present in EE membranes. We identify the domain of VP3 involved in PI3P-binding and its role in viral replication. Finally, our molecular simulations results unveil a two-stage modular mechanism for VP3 association with EE. Firstly, the carboxy-terminal region of VP3 adsorbs to the membrane via non-specific electrostatic interactions. Then, in the second stage, the VP3 core seals the membrane engagement by specifically binding PI3P through its P2 domain, additionally promoting PI3P accumulation.