DIETARY SUPPLEMENTATION WITH INTEGRAL CHIA AND FLAX FLOURS AMELIORATES SYSTEMIC INFLAMMATION

COLUSSI, NARELLA; TODARO, JUAN SANTIAGO; RODRIGUEZ JUAN PABLO; OLEA, GABRIELA; FERRINI, LEANDRO; STOYANOFF, TANIA ROMINA; AGUIRRE, MARÍA VICTORIA

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2023

0025-7680

ntroduction: Chia and flax seeds are rich in alpha-linolenic acid (ALA), which is bioconverted into theactive derivatives eicosapentaenoic (EPA) and doco-sahexaenoic (DHA) having multiple beneficial effects.However, there is limited knowledge about the anti-inflammatory effects of chia and flax integral floursdiets rich in ALA.Objective: The study aimed to evaluate the anti-inflammatory effect of dietary supplementation withintegral chia and flax flours in a murine model of LPS-induced systemic inflammation.Methods: Balb/c mice were distributed into threegroups: diet A (control), diet B (supplemented with inte-gral chia flour), and diet C (supplemented with integralflax flour). Nutritional, hematological, and biochemicaldeterminations were performed. ALA, EPA, and DHAwere assessed by GC-MS in the liver, brain, cardiacand skeletal muscles. NF-kB immunoassays were per-formed in kidney, liver, and peritoneal macrophages,respectively. The phagocytic capacity was determinedin peritoneal macrophages and the expression of thepro- and anti-inflammatory cytokines was assessed byRT-qPCR in the kidney, liver, and spleen.Results: Diets B and C exhibited optimal nutritionaladequacy and caused increased levels of ALA, EPA, andDHA in critical tissues compared to the control. Thephagocytic capacity of murine peritoneal macrophages(p< 0.01) and IL-10 transcription increased, whereas theexpression of NF-κB, IL-1β, IL-6, and TNF-α decreased inanimals fed both experimental diets.Conclusions: This work contributes to the currentknowledge of the anti-inflammatory effects of chia andflax integral flours rich in ALA and reinforces the healthadvantages of their consumption.