ASPARAGINE QUANTIFICATION IN CELLULAR CULTURE MEDIA USING COPPER MODIFIED CARBON NANOTUBES COMPOSITE ELECTRODES

MARIA LORENA LÓPEZ RODRIGUEZ; GUILLERMINA L. LUQUE; CARMELO J. FELICE; NANCY F. FERREYRA; ROSSANA E. MADRID; GUSTAVO A. RIVAS; CARLA E. GIACOMELLI

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Año: 2011 vol. 158 p. 423 - 426

0925-4005

Direct quantification of Asparagine (Asp) in a Streptomyces sp. M7 (SM7) culture medium was performed using multi-wall carbon nanotubes composite electrodes modified with copper microparticles (CNTPECu). A sensitive and fast response is achieved at CNTPE-Cu after additions of SM7 cultures supernatant, without interference of the culture medium components. This response allows determining the Asp concentration as a function of the bacteria growth. The results reveal that the SM7 bacteria consume Asp as a carbon and nitrogen sources in the absence of another carbon source such as glucose.