Epigenetic impact of hypothyroidism on the functional differentiation of the mammary gland in rats

FIORELLA CAMPO VERDE ARBOCCO; LOURDES INÉS PASCUAL; PASCUAL DAIANA GARCÍA; IRINA ORTIZ; CARLOS D GAMARRA-LUQUES; CARON RUBEN; HAPON M BELEN

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2024

0303-7207

Mammary gland (MG) lactogenic differentiation involves epigenetic mechanisms. Wehave previously shown that hypothyroidism (HypoT) alters the MG transcriptome inlactation. However, the role of thyroid hormones (T3 and T4 a.k.a. THs) in epigeneticdifferentiation of MG is still unknown. We used a model of post-lactating HypoT rats tostudy in MG: a) Methylation and expression level of Gata3, Elf5, Stat6, Stat5a, Stat5b;b) Expression of Lalba, IL-4Rα and Ncoa1 mRNA; c) Histone H3 acetylation and d)Estrogen and progesterone concentration in serum. HypoT increases the estrogenserum level, decreases the progesterone level, promotes methylation of Stat5a, Stat5band Stat6, decreasing their mRNA level and of its target genes (Lalba and IL-4Rα) andincreases the Ncoa1 mRNA expression and histone H3 acetylation level. Our resultsproved that HypoT alters the post-lactation MG epigenome and could compromisemammary functional differentiation.