Histological and inmunihistochemical description from gonadal development in a neotropical insectivorous bat Eumops patagonicus

RODRIGUEZ, FLORENCIA EVELIN; OLEA, GABRIELA BEATRIZ; AGUIRRE, MARÍA VICTORIA; LOMBARDO, DANIEL MARCELO

ANIMAL REPRODUCTION SCIENCE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2024

0378-4320

The order Chiroptera is one of the most diverse orders in terms of the numberof species, however, very few studies have been conducted in this groupregarding its embryonic development. In this area, studies have focused onstaging through morphological characters, but those describing organogenesisare scarce. Therefore, this work describes the gonadogenesis of Eumopspatagonicus a Sudamerican insectivorous bat, with an emphasis on ovariandevelopment and determination of the migration stage of the primordial germcells (PGCs) through immunostaining. Pregnant females of this species werecaptured to carry out the study, and the embryos were processed to obtainhistological sections using the conventional histology technique andimmunohistochemistry (OCT4, PCNA, Bax and Bcl-XL).Critical stages of gonadal differentiation were described, starting from theformation of the urogenital ridge and the identification to PCGs with thepositive reaction for OCT4 in embryological development stadio 13. At stadio17 to development, it is observed an undifferentiated gonad with activeproliferation and then a defined testis (S. 21) and ovaries (S.23 and S.25). Theembryo ovaries S.23 show a cortex formed for ovogonia cyst/nets that have acraniocaudal development whit difference in the immunolabeling to PCNA, Baxand Bcl-XL. The embryo ovaries S.25 show the primordial follicles in the ovarycortex. The results determined that there is a conserved pattern in embryonicdevelopment in this order when comparing E. patagonicus with frugivorous bat.This information is important to the establishment of relations in developmentalin mammalian and into the order Chiroptera.