The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells

CLAUDIA SOLARI; MARIA VICTORIA PETRONE; AYELEN TORO; CAMILA VÁZQUEZ ECHEGARAY; MARIA SOLEDAD COSENTINO; ARIEL WAISMAN; MARCOS FRANCIA; LINO BARAÑAO; SANTIAGO MIRIUKA; ALEJANDRA GUBERMAN

BMC Research Notes

Biomed Central

Año: 2019

1756-0500

Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes and low levels of oxidative stress. Although the expression and activity of antioxidant enzymes are reduced throughout the differentiation, the knowledge about the transcriptional regulation of genes involved in defense against oxidative stress is yet restricted. Since glutathione is a central component of a complex system involved in preserving cellular redox status, we aimed to study whether the expression of the glutathione reductase (Gsr) gene, which encodes an essential enzyme for cellular redox homeostasis, is modulated by the transcription factors critical for self-renewal and pluripotency of ESCs.