Eco-friendly approaches of mycosynthesized copper oxide nanoparticles (CuONPs) using Pleurotus citrinopileatus mushroom extracts and their biological applications

KUMAR MANIMARAN; KUMAR SELVARAJ

Environmental Research

Academic Press Inc.

Año: 2023 vol. 232 p. 116307 - 116319

This current study aims to develop a unique biomaterial that can fight against oxidative stress and microbialinfections without causing any harm. As a result, an easy-to-make, environment-friendly, long-lasting, and nontoxiccopper oxide nanoparticle (CuONP) was synthesized using an edible mushroom Pleurotus citrinopileatusextract. The UV–vis spectroscopy analyses reflected a sharp absorbance peak at 250 nm. The FTIR, XRD, SEM,HR-TEM, and EDX instrumental tools were used to characterize the myco-produced CuONPs. The face-centredcubic (FCC) CuONPs were found to have diffraction peaks at the planes of (110), (002), (111), (112), (020),(202), (113), (310), (220), and (004). The HR-TEM result showed the particles had a spherical structure and anaverage nanoparticles size of 20 nm. The antimicrobial activity results expressed the broad spectrum of antibacterialeffect and the better growth inhibition zone was recorded in P. aeruginosa (8.3 ± 0.1), E. coli (7.4 ±0.3), K. pneumoniae (7.2 ± 0.1), S. aureus (7.1 ± 0.3), S. pneumoniae (6.3 ± 0.2), and B. cereus (6.2 ± 0.3 mm).The cytotoxicity efficacy of myco-synthesized CuONPs tested against a cancer cell line (HT-29) observed the bestresult in low doses of mushroom extract (45.62 μg/mL). Based on the outcome of the study suggests that themycosynthesized CuONPs using Pleurotus mushroom extract might serve as an alternative agent for biomedicalapplications in the near future.