PERSONAL DE APOYO
BURDISSO Paula
artículos
Título:
Second double-stranded RNA binding domain of dicer-like ribonuclease 1: structural and biochemical characterization.
Autor/es:
BURDISSO, PAULA; SUAREZ, IRINA; BOLOGNA, NICOLAS G.; PALATNIK, JAVIER F.; BERSCH, BEATE; RASIA, RODOLFO M.
Revista:
BIOCHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2012 vol. 51 p. 10159 - 10166
ISSN:
0006-2960
Resumen:
Dicer-like ribonuclease III enzymes are involved in different paths related to RNA silencing in plants. Little is known about the structural aspects of these processes. Here we present a structural characterization of the second double-stranded RNA binding domain (dsRBD) of DCL1, which is presumed to participate in pri-micro-RNA recognition and subcellular localization of this protein. We determined the solution structure and found that it has a canonical fold but bears some variation with respect to other homologous domains. We also found that this domain binds both doublestranded RNA and double-stranded DNA, in contrast to most dsRBDs. Our characterization shows that this domain likely has functions other than substrate recognition and binding.