Modulation of gut immune response by probiotic fermented milk consumption to control IgE in a respiratory allergy model

VELEZ, E.M.; NOVOTNY NUÑEZ, I; CORREA S.; MALDONADO GALDEANO, CAROLINA; PERDIGÓN, GABRIELA

Beneficial Microbes

Wageningen Academics Publishers

Año: 2021 vol. 12 p. 175 - 186

Allergies are a world increasing health issue; most treatments are oriented to alleviate symptoms.3 Probiotics have health benefits improving immune responses. In previous work we found that4 commercial probiotic fermented milk (PFM) significantly reduced specific-IgE levels in serum5 increasing specific-IgG and controlled allergic response in an adult mouse respiratory allergy model to 6 ovalbumin (OVA). Here we continue our study determining the mechanism triggered in the gut by the 7 PFM ingestion that influence the diminution of IgE previously reported. Five different groups of 8 BALB/c mice were assessed: normal-control, basal (drinks PFM five days without OVA sensitization), 9 sensitization-control (no PFM intake), previous and continuous-PFM administration. Allergen 10 administration: 3 OVA injections (1% in PBS) followed by aerosols exposure for 7 days. 11 Determinations: total S-IgA and cytokines in small intestine-(SI) fluid; CD11b+, CD103+, IgA+ cells and 12 cytokine producing cells were studied in SI. In lungs we analysed co-expression of CD4 with IFN-γ or 13 IL-10, IgE+ cells and IL-12 quantification. Results: the continuous intake of PFM increased the 14 expression of CD103 marker and decreased pro-inflammatory cytokines. Co-expression of CD4 and 15 IFN-γ was confirmed in lungs from continuous PFM intake in parallel with less count of IgE+ cells and 16 higher IL-12 concentration. PFM consumption induces signalling from the gut to the lungs by increasing 17 CD103+ dendritic cells expression related with regulatory mechanisms, controlling the IgE level 18 reported previously. We demonstrated that PFM intake induced a clear balance to Th1 response 19 preventing Th2 allergy response. PFM could be a good strategy to alleviate allergy development