Effects of the addition of insulin-transferrin-selenium (ITS) and/or metformin to the in vitro maturation of porcine oocytes on cytoplasmic maturation and embryo development

LUCHETTI MC; LORENZO S; ELIA EM; TEPLITZ GM; CRUZANS PR; CAROU MC; LOMBARDO DM

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2022

1031-3613

Context: One of the main problems of porcine in vitro maturation (IVM) is incompletecytoplasmatic maturation. Nuclear and cytoplasmic maturation will determine the futuresuccess of fertilisation and embryo development. Insulin-transferrin-selenium (ITS) hasinsulin-like and antioxidant effects, and metformin (M) is an insulin-sensitizer andantioxidant drug. Aims: To assess the effects of adding ITS and/or M in porcine IVMmedia on cytoplasmic maturation and early embryo development. Methods: Cumulus-oocyte complexes (COC) were IVM with M (10-4M), ITS (0.1% v/v), M+ITS or no adding(Control). Key results: ITS increased glucose consumption compared to Control and M(P< 0.01), and M+ITS did not differ from ITS or Control. Redox balance: M, ITS andM+ITS increased glutathione (P< 0.01) and decreased lipid peroxidation (P< 0.005). Theviability of cumulus cells by flow cytometry increased with M (P< 0.005) and decreasedwith ITS (P< 0.001); M+ITS did not differ from Control. After IVF, M increased penetrationand decreased male pronucleus (P< 0.05). Embryo development: cleavage increasedwith M (P< 0.05), and blastocysts increased with ITS and M+ITS (P< 0.05). The numberof blastocyst cells increased with ITS (P< 0.05). Conclusions: Adding ITS and M+ITS toporcine IVM media benefits embryo development to blastocysts, but ITS alone has bettereffects than M+ITS.