In vitro blastocyst implantation and trophoblast migration are 1 disrupted by the UV filter benzophenone-3 (BP3).

ABUD, JULIÁN; PAGOTTO, ROMINA; GALLIANI, VALENTINA; TEGLIA CM; CULZONI MJ; BOLLATI-FOGOLÍN, MARIELA; ZENCLUSSEN, MARÍA LAURA; RODRÍGUEZ HORACIO ADOLFO*

ENVIRONMENTAL POLLUTION

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2023

0269-7491

Benzophenone-3 (BP3), a common ingredient in personal care products (PCPs) due to 22 its well-established effectiveness in blocking UV radiation. Sunscreen products are 23 among the most widely used PCPs-containing BP3 applied to the skin, resulting in 24 significant human exposure to BP3 primarily through a dermal application. Previously, 25 we found that BP3 causes fetal growth retardation (FGR) in mice when it is dermally 26 administered in the first days of gestation. In the present work, we have tested the action 27 of BP3 using an in vitro model of anchoring and implantation of murine blastocysts as 28 well as by analyzing the migration ability of the human trophoblast cell line Swan 71. 29 BP3 concentrations used in our experiments were: a) 2 μg/L, based on the predicted no-302effect concentration for aquatic environments; b) 20 μg/L, similar to the levels of BP3 31 detected in serum and amniotic fluid of pregnant mice exposed to BP3 from gestation 32 day (gd) 0 to gd 6 by dermal exposure; and c) BP3-200 μg/L, based on plasma 33 concentrations reached by BP3 after a whole body topical application of sunscreens in 34 humans. We found that BP3 negatively affected critical parameters of both in vitro 35 implantation and early embryo development and reduced trophoblast migration through 36 the androgen receptor (AR) pathway. Based on these findings, it can be concluded that 37 BP3 can affect early in vitro embryo implantation and trophoblast migration capacity.