INVESTIGADORES
LAROCCA Maria Cecilia
artículos
Título:
Adaptive hepatic changes in mild stenosis
Autor/es:
EMILIO A. RODRIGUEZ GARAY; CECILIA LAROCCA; GERARDO PISANI; MARÍA DEL LUJÁN ALVAREZ; GRACIELA P. RODRIGUEZ
Revista:
Research in Experimental Medicine
Editorial:
Springer-Verlag
Referencias:
Año: 1999 vol. 198 p. 307 - 323
Resumen:
Adaptive hepatic changes were investigated in rats with mild stenosis
of the common bile duct and in sham-operated controls. The studies were
performed 24 h and 7?12 days postoperatively. A continuous intravenous infusion
of taurocholic acid at stepwise-increasing rates was performed to explore
the responses to bile acid effects. During the infusion, bile flow and the
outputs of bile acids, phospholipids, cholesterol, alkaline phosphatase and
gamma glutamyl transpeptidase were studied. At the end of the infusion, hepatic
morphometric measurements were performed. In other experimental sets,
biliary excretions of horseradish peroxidase, a marker of microtubule-dependent
vesicular transport in the hepatocyte, and sulphobromophthalein, a wellknown
organic anion model, were studied. In other rats, bile acid pool size and
composition were determined by depletion of bile. The results in rats with mild
stenosis maintained for 24 h showed a greater susceptibility to the toxicity of
taurocholic acid, as revealed by the abrupt decrement in bile flow at high rates
of infusion, and increased outputs of phospholipids and canalicular enzymes.
Conversely, rats with mild stenosis maintained for 7?12 days showed decreased
bile acid maximum secretory rate and biliary outputs of phospholipids
and canalicular enzymes, as well as hepatocyte hypertrophy. These findings
may explain the limited hepatic and systemic repercussion of experimental
mild stenosis of the common bile duct and help us to understand the early
stages of constriction of the common bile duct in man.