INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Interference-free analysis using three-way fluorescence data and the parallel factor model. Determination of fluoroquinolone antibiotics in human serum
Autor/es:
MUÑOZ DE LA PEÑA, A; ESPINOSA MANSILLA, A; GONZALEZ GOMEZ, D; AC OLIVIERI,; GOICOECHEA, HÉCTOR C
Revista:
ANALYTICAL CHEMISTRY
Editorial:
American Chemical Society
Referencias:
Año: 2003 vol. 75 p. 2640 - 2646
ISSN:
0003-2700
Resumen:
Three-way fluorescence data and multivariate calibration
based on parallel factor analysis (PARAFAC) are combined
for the simultaneous quantitation of three fluoroquinolone
anitibiotics (norfloxacin, enoxacin, and ofloxacin)
in human serum samples. The three analytes can
be adequately determined with limits of detection of 0.2,
3.0, and 0.5 íg L-1, respectively, with minimum experimental
effort. The selected analytical methodology fully
exploits the so-called second-order advantage of the
employed three-way data, allowing obtaining individual
concentrations of calibrated analytes in the presence of
any number of uncalibrated (serum) components. In
contrast to PARAFAC, less satisfactory results were
obtained with a multidimensional partial least-squares
(nPLS) model trained with the same calibration set.nPLS) model trained with the same calibration set.íg L-1, respectively, with minimum experimental
effort. The selected analytical methodology fully
exploits the so-called second-order advantage of the
employed three-way data, allowing obtaining individual
concentrations of calibrated analytes in the presence of
any number of uncalibrated (serum) components. In
contrast to PARAFAC, less satisfactory results were
obtained with a multidimensional partial least-squares
(nPLS) model trained with the same calibration set.nPLS) model trained with the same calibration set.