INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Enhanced Synchronous Spectrofluorometric Determination of Tetracycline in Blood Serum by Chemometric Analysis. Comparison of Partial Least-Squares (PLS-1) and Hybrid Linear Analysis (HLA) Calibrations
Autor/es:
GOICOECHEA, HÉCTOR C; AC OLIVIERI,
Revista:
ANALYTICAL CHEMISTRY
Editorial:
American Chemical Society
Referencias:
Año: 1999 vol. 71 p. 4361 - 4368
ISSN:
0003-2700
Resumen:
Tetracycline has been determined in human serum samples by a combination of: (1) synchronous fluorescence spectra of whole sera treated with Mg2+, and (2) the multivariate calibration methods of partial leastsquares (PLS-1) and a variant of the recently introduced hybrid linear analysis (HLA), which does not require the knowledge of pure-component spectra. The calibration set was designed with 50 sera spiked with concentrations of tetracycline in the range 0.0-4.0 íg mL-1. Studies concerning validation, precision, accuracy and figures of merit (selectivity, sensitivity and limit of determination) were also carried out. A novel wavelength-selection procedure was applied to minimize the effect of nonmodeled interferents present in serum samples containing bilirubin, triglycerides, and salicylate. Overall, the performance of the newly developed HLA approach seems to be better than that of PLS-1.2+, and (2) the multivariate calibration methods of partial leastsquares (PLS-1) and a variant of the recently introduced hybrid linear analysis (HLA), which does not require the knowledge of pure-component spectra. The calibration set was designed with 50 sera spiked with concentrations of tetracycline in the range 0.0-4.0 íg mL-1. Studies concerning validation, precision, accuracy and figures of merit (selectivity, sensitivity and limit of determination) were also carried out. A novel wavelength-selection procedure was applied to minimize the effect of nonmodeled interferents present in serum samples containing bilirubin, triglycerides, and salicylate. Overall, the performance of the newly developed HLA approach seems to be better than that of PLS-1.-4.0 íg mL-1. Studies concerning validation, precision, accuracy and figures of merit (selectivity, sensitivity and limit of determination) were also carried out. A novel wavelength-selection procedure was applied to minimize the effect of nonmodeled interferents present in serum samples containing bilirubin, triglycerides, and salicylate. Overall, the performance of the newly developed HLA approach seems to be better than that of PLS-1.