INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Enhanced Synchronous Spectrofluorometric Determination of Tetracycline in Blood Serum by Chemometric Analysis. Comparison of Partial Least-Squares (PLS-1) and Hybrid Linear Analysis (HLA) Calibrations
Autor/es:
GOICOECHEA, HÉCTOR C; AC OLIVIERI,
Revista:
ANALYTICAL CHEMISTRY
Editorial:
American Chemical Society
Referencias:
Año: 1999 vol. 71 p. 4361 - 4368
ISSN:
0003-2700
Resumen:
Tetracycline has been determined in human serum
samples by a combination of: (1) synchronous fluorescence
spectra of whole sera treated with Mg2+, and (2)
the multivariate calibration methods of partial leastsquares
(PLS-1) and a variant of the recently introduced
hybrid linear analysis (HLA), which does not require the
knowledge of pure-component spectra. The calibration set
was designed with 50 sera spiked with concentrations of
tetracycline in the range 0.0-4.0 íg mL-1. Studies
concerning validation, precision, accuracy and figures of
merit (selectivity, sensitivity and limit of determination)
were also carried out. A novel wavelength-selection procedure
was applied to minimize the effect of nonmodeled
interferents present in serum samples containing bilirubin,
triglycerides, and salicylate. Overall, the performance
of the newly developed HLA approach seems to be better
than that of PLS-1.2+, and (2)
the multivariate calibration methods of partial leastsquares
(PLS-1) and a variant of the recently introduced
hybrid linear analysis (HLA), which does not require the
knowledge of pure-component spectra. The calibration set
was designed with 50 sera spiked with concentrations of
tetracycline in the range 0.0-4.0 íg mL-1. Studies
concerning validation, precision, accuracy and figures of
merit (selectivity, sensitivity and limit of determination)
were also carried out. A novel wavelength-selection procedure
was applied to minimize the effect of nonmodeled
interferents present in serum samples containing bilirubin,
triglycerides, and salicylate. Overall, the performance
of the newly developed HLA approach seems to be better
than that of PLS-1.-4.0 íg mL-1. Studies
concerning validation, precision, accuracy and figures of
merit (selectivity, sensitivity and limit of determination)
were also carried out. A novel wavelength-selection procedure
was applied to minimize the effect of nonmodeled
interferents present in serum samples containing bilirubin,
triglycerides, and salicylate. Overall, the performance
of the newly developed HLA approach seems to be better
than that of PLS-1.