Effects of dietary canthaxanthin on ultraviolet radiation stress on prawn (Artemesia longinaris)

ARZOZ, NATALIA SOLEDAD; MARCOVAL, MARIA ALEJANDRA; DÍAZ, ANA CRISTINA; ESPINO, MARIA LAURA; VELURTAS, SUSANA M.; FENUCCI, JORGE LINO

Marine and Fishery Sciences

Instituto Nacional de Investigación y desarrollo pesquero

Lugar: Mar del Plata; Año: 2023

2683-7951

The aims of this research were to investigate the effects of diets added with synthetic canthaxanthin (10 % parafarm) and evaluate its possible protective role under ultraviolet radiation (UVR) in prawn Artemesia longinaris. Three isoproteic and isolipidic diets (41% protein and 12 % lipid) with 0 (C0), 100 (C100), and 300 (C300) mg of canthaxanthin/kg of diet, were prepared. Before the radiation experiment, to determine a possible accumulation of carotenoids, prawns were fed different diets for 21 days. Subsequently, the animals were exposed to two radiation treatments for 7 days: a) photosynthetically active radiation (PAR, 400-700 nm); b) total radiation (PAR+UVR, 280-700 nm). In animals exposed to PAR+UVR treatment, survival varied between 50 and 83.33% with the highest value in animals fed diet C300. At the end of the experiment, significant statistical differences were registered in integument carotenoid concentration. Under UVR stress, the highest decrease in non-polar carotenoid and esterified astaxanthin were recorded in prawns fed diets with canthaxanthin. The scavenging properties were evaluated by electron resonance spectroscopy (EPR) using the stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. Prawns fed with C300 showed the greatest activity to quench DPPH. The results suggest that dietary canthaxanthin could be acting as an antioxidant against reactive oxygen species and produced high tolerance under UVR stress.