Three-dimensional culture model of tumoral cell

PALMA MARÍA BELÉN; MARTINEZ MARINA; LUZZANI CARLOS; ANDRINI LAURA; ERRECALDE ANA LÍA; INDA ANA MARIA; PELINSKI PABLO; MIRIUKA SANTIAGO; GARCIA MARCELA

international Journal of Cell Science & Molecular Biology

Juniper publishes

Lugar: Newbury Park; Año: 2018

2572-1100

The mammospheres are a multicellular tumor spheroid model. These are a widely used culture system to mimic the three-dimensionality of tumors. In this manuscript, we narrate the development the model using a murine mammary adenocarcinoma, which we used to study tumor physiology and therapeutic applications. We assay three techniques published in previous works. The best condition was used V-shaped 96-well plates with an agarose monolayer because we were obtained compact and robust spheroids. After that, we studied the interaction between tumoral cells and mesenchymal stem cells (MSC) derived to human umbilical cord in three dimensional culture. Additionally, for evaluate spheroid´s structure by optical microscopy we performed histological staining of the mammospheres with hematoxylin-eosin. Moreover, we made immunohistochemistry staining to evaluate cell proliferation index and neovascularization, with Brdu and VEGF, respectively. We found that the presence of MSC could modify the tumoral cells. We can conclude that mamospheres can be perform with more than one cell type similarly to what happens in a in vivo grafted tumor and this can be used to study the tumor physiology and therapeutic agents on solid tumors without the use of laboratory animals.