INVESTIGADORES
MORON Victor Gabriel
artículos
Título:
Orally Administered OVA/CpG-ODN Induces Specific Mucosal and Systemic Immune Response in Young and Aged Mice
Autor/es:
DIEGO ALIGNANI; BELKYS MALETTO; MIRIAM LISCOVSKY; ANDREA RÓPOLO; GABRIEL MORÓN; MARÍA C. PISTORESI-PALENCIA
Revista:
JOURNAL OF LEUKOCYTE BIOLOGY
Editorial:
FEDERATION AMER SOC EXP BIOL
Referencias:
Año: 2005 vol. 77 p. 898 - 905
ISSN:
0741-5400
Resumen:
We have previously demonstrated that
subcutaneously administered ovalbumin (OVA)
plus synthetic oligodeoxynucleotides containing
immunostimulatory CpG motifs (CpG-ODN) as adjuvant
stimulate cellular and humoral immunity
and promote T helper cell type 1 differentiation in
aged mice. The present study assessed the ability of
CpG-ODN to induce an OVA-specific immune response
after oral immunization in young (3-monthold)
and aged (18-month-old) BALB/c mice. Oral
OVA/CpG-ODN immunization induces a similar
OVA-specific T cell-proliferative response (in mucosal
and systemic tissues), immunoglobulin G
(IgG) in plasma, and IgA in intestinal washes in both
groups of ages. The OVA-specific humoral immune
response observed in aged mice was similar to the
one observed in young mice, peaking at day 7 after
the last oral immunization and was present over 40
days after the last oral immunization. The pattern
of cytokines released in culture supernatants in
both groups of mice was similar, with specific interferon-
secretion in the absence of interleukin-
5 responses. These results provide evidence
that orally administered OVA/CpG-ODN induces a
young-like, specific, immune response against OVA
in aged mice, showing that CpG-ODN might be
used as a mucosal adjuvant during aging.
secretion in the absence of interleukin-
5 responses. These results provide evidence
that orally administered OVA/CpG-ODN induces a
young-like, specific, immune response against OVA
in aged mice, showing that CpG-ODN might be
used as a mucosal adjuvant during aging.