The combined treatment of Insulin and Naringin improves bone properties in rats with type 1 diabetes mellitus

RODRIGUEZ, VALERIA; PICOTTO, GABRIELA; RIVOIRA, MARÍA; RIGALLI, ALFREDO; TOLOSA DE TALAMONI, NORI

APPLIED PHYSIOLOGY, NUTRITION AND METABOLISM

NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Año: 2024

1715-5312

We have studied the effects of individual and combined treatment of Insulin (I) and Naringin (NAR) on the bone structure and biomechanical properties of femurs from streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were divided into 5 groups: 1) controls, 2) STZ-induced diabetic rats, 3) STZ-induced diabetic rats treated with I, 4) STZ-induced diabetic rats treated with NAR, and 5) STZ-induced diabetic rats treated with I+NAR. Bone mineral density (BMD), bone histomorphometry, biomechanical testing, bone biomarker expressions were accomplished in femur of all animals, as well as serum biochemical analyses. The combined treatment of I+NAR increased the body weight and the femur BMD from STZ-induced diabetic rats. The bone biomechanical properties and the bone morphology of the femurs from STZ-induced diabetic rats were also improved by the combined treatment. The increased number of osteoclasts in STZ-induced diabetic rats was partially prevented by I, NAR or I+NAR. NAR or I+NAR completely blocked the decrease in the number of osteocalcin (+) cells in the femur from STZ-induced diabetic rats. RUNX2 immunostaining was much lower in STZ-induced diabetic rats than in control animals; the combination of I+NAR totally blocked this effect. The combined treatment not only ameliorated bone quality and function, but also normalized the variables related to glucose metabolism. Therefore, the combination of I+NAR might be a better therapeutic strategy than the individual I or NAR administration to reduce bone complications in diabetic patients.