Effects of the addition of insulin-transferrin-selenium (ITS) and/or metformin to the in vitro maturation of porcine oocytes on cytoplasmic maturation and embryo development

LUCHETTI, CAROLINA GRISELDA; LORENZO, MARÍA SOLEDAD; ELIA, EVELIN MARIEL; TEPLITZ, GABRIELA MAIA; CRUZANS, PAULA ROMINA; CAROU, MARÍA CLARA; LOMBARDO, DANIEL MARCELO

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Año: 2023 vol. 35 p. 363 - 374

1031-3613

CONTEXT: One of the main problems of porcine in vitro maturation (IVM) is incomplete cytoplasmatic maturation. Nuclear and cytoplasmic maturation will determine the future success of fertilisation and embryo development. Insulin-transferrin-selenium (ITS) has insulin-like and antioxidant effects, and metformin (M) is an insulin-sensitiser and antioxidant drug. AIMS: To assess the effects of adding ITS and/or M in porcine IVM media on cytoplasmic maturation and early embryo development. METHODS: Cumulus -oocyte complexes (COC) were IVM with M (10-4 M), ITS (0.1% v/v), M+ITS or no adding (Control). KEY RESULTS: ITS increased glucose consumption compared to Control and M (P