Low molecular weight hyaluronan preconditioning of tumor-pulsed dendritic cells increases their migratory ability and induces immunity against murine colorectal carcinoma

ALANIZ L; RIZZO M; GARCÍA MG; AQUINO JB; MALVICINI M; ATORRASAGASTI C; BAYO J; ECHEVERRIA I; SAROBE P; MAZZOLINI G

CANCER IMMUNOLOGY IMMUNOTHERAPY

SPRINGER

Año: 2011 p. 1383 - 1395

0340-7004

We have recently shown that systemic administration of low molecular weight hyaluronan (LMW HA) significantly reduces colorectal carcinoma (CRC) growth in vivo. The elicited response is partially mediated by activated dendritic cells (DC). To potentiate the ability ofDC loaded with whole tumor lysate (DC/TL) to induce immunity against CRC in mice, we aimed to study the effects of preconditioning DC with LMW HA for therapeutic vaccination. LMW HA improved maturation of ex vivo generated DC, increased IL-12, decreased IL-10 production, and enhanced a MLR activity in vitro. Although TNF-a showed a similar capacity to mature DC, preconditioning of DC/TL with LMW HA increased theirability to migrate in vitro toward CCL19 and CCL-21 in a CD44- and a TLR4-independent manner; this effect was superior to Poly(I:C), LPS, or TNF-a and partially associated with an increase in the expression of CCR7.