INVESTIGADORES
REPETTO Marisa Gabriela
artículos
Título:
New technique to quantify phospolipids in disordered phase in lung surfactant by electron spin resonance
Autor/es:
CIMATO, ALEJANDRA; FACORRO, GRACIELA; REPETTO, MARISA; MARTÍNEZ SARRASAGUE, MARGARITA
Revista:
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2023 vol. 316
ISSN:
1569-9048
Resumen:
In pulmonary surfactant (PS) the coexistence of the ordered (Lo) and disordered (Ld) lipid phases would be essential for an optimal activity. Electron spin resonance (ESR) of PS labeled with 5-doxil stearic acid shows two spectral components called S and W. S/W ratio could be understood as the ratio between the probe population in Lo and in Ld. Although the specificity of S/W as an indicator of Lo/Ld has not yet been demonstrated, S/W has been used qualitatively to study changes in Lo/Ld. The goal of this paper is to stablish the correlation between S/W parameter and the amount of lipids in Ld (%Ld) measured by the ESR TEMPO technique described in our previous work.S/W and %Ld were measured in different PS samples under different experimental conditions. The results showed an inverse correlation between S/W and %Ld (r = −0.983; p < 0.001). We demonstrated that the S/W is sensible to the changes of Lo/Ld and can be used to quantify the %Ld.