INVESTIGADORES
SÜLSEN valeria Patricia
artículos
Título:
Antiproliferative effect and ultrastructural alterations induced by psilostachyin on Trypanosoma cruzi
Autor/es:
SÜLSEN V; BARRERA P; MUSCHIETTI L; MARTINO V; SOSA M
Revista:
MOLECULES
Editorial:
MDPI Publishing
Referencias:
Lugar: Basel, Switzerland; Año: 2010 vol. 15 p. 545 - 553
ISSN:
1420-3049
Resumen:
The effect of psilostachyin, a natural sesquiterpene lactone, on the growth and
viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported. The
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported. The
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported. The
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.
antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and
measuring their viability by using the dye exclusion technique. The effect on parasite
growth was irreversible at concentrations higher than 1.0 ug/mL and the addition of
glutathione only partially blocked the effect of the compound. Moreover, we have studied
the effects of this natural compound on parasite ultrastructure by transmission electron
microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites
at a concentration of 0.5 ug/mL, with important mitochondrial swelling and deformity of
the kinetoplast.