INVESTIGADORES
PRECIADO Maria Victoria
artículos
Título:
MicroRNA signature from extracellular vesicles of HCV/HIV co-infected individuals differs from HCV mono-infected
Autor/es:
CAIROLI VICTORIA; DANIEL VALLE-MILLARES; MARÍA C. TERRÓN; DANIEL LUQUE ; PABLO RYAND; LOURDES DOMINGUEZ; LUZ MARTÍN-CARBONERO; IGNACIO DE LOS SANTOS; ELENA DE MATTEO; BEATRIZ AMEIGEIRAS; VERÓNICA BRIZ; PAOLA CASCIATO; PRECIADO MV *EQUAL CONTRIBUTOR; PAMELA VALVA *EQUAL CONTRIBUTOR; AMANDA FERNÁNDEZ RODRIGUEZ *EQUAL CONTRIBUTOR
Revista:
JOURNAL OF MOLECULAR MEDICINE (BERLIN, GERMANY)
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2023
ISSN:
0946-2716
Resumen:
Hepatitis C virus (HCV) coinfection with Human Immunodeficiency Virus (HIV) has a detrimental impact on disease progression. Increasing evidence points to extracellular vesicles (EVs) as important players of the host-viral cross-talk. The microRNAs (miRNAs), as essential components of EVs cargo, are key regulators of normal cellular processes and also promote viral replication, viral pathogenesis and disease progression. We aimed to characterize the plasma derived-EVs miRNA signature of chronic HCV infected and HIV coinfected patients to unravel the molecular mechanisms of coinfection. EVs were purified and characterized from 50 plasma samples (21 HCV mono- and 29 HCV/HIV co-infected). EV-derived small RNAs were isolated and analysed by massive sequencing. Known and de novo miRNAs were identified with miRDeep2. Significant differentially expressed (SDE) miRNA identification was performed with generalized linear models and their putative dysregulated biological pathways were evaluated. Study groups were similar for most clinical and epidemiological characteristics. No differences were observed in EVs size or concentration between groups. Therefore, HCV/HIV co-infection condition did not affect the concentration or size of EVs but produced a disturbance in plasma derived-EVs miRNA cargo. Thus, a total of 149 miRNAs were identified (143 known and 6 de novo) leading to 37 SDE miRNAs of which 15 were upregulated and 22 downregulated in HCV/HIV co-infected patients. SDE miRNAs regulate genes involved in inflammation, fibrosis and cancer, modulating different biological pathways related to HCV and HIV pathogenesis. These findings may help to develop new generation biomarkers and treatment strategies, in addition to elucidate the mechanisms underlying virus-host interaction.