INVESTIGADORES
ORTEGA Hugo Hector
artículos
Título:
Oestradiol Induced Inhibition of neuroendocrine Markers Expression in Leydig cells of adult rats
Autor/es:
ORTEGA, HH; R SALVETTI, NATALIA; CELINA BARAVALLE,; A LORENTE, JUAN; A MIRA, GUSTAVO
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Referencias:
Año: 2006 vol. 41 p. 204 - 209
ISSN:
0936-6768
Resumen:
The objectives of this work were to determine the changes in
the expression of neuroendocrine markers in Leydig cell by
oestradiol treatment, and to determine whether testosterone is
able to recover partially the effects of hormonal suppression
induced by oestradiol. Adult male rats were injected daily with
either 50 lg of oestradiol or oestradiol plus testosterone
propionate (25 mg every 3 days) for 15 days. The animals
were sacrificed and testicles were dissected and processed by
routine histological protocols. FSH and LH serum levels were
determined by radioimmunoassay. The visualization of antigens
was achieved by the streptavidin-peroxidase immunohistochemical
method. Antibodies against chromogranin A
(CrA), S-100 protein (S-100), P substance (PS), synaptofisin
(SYN), neurofilament protein (NF), gliofibrillary acidic protein
(GFAP) and neuron specific enolase (NSE) were used. The
mean LH and FSH serum concentrations were consistently
suppressed with hormonal treatments. Intermediate filaments
(NF and GFAP) showed no difference in their expression. The
expression of S-100, NSE and SYN was significantly lower in
both hormone-treated groups. In oestradiol-treated rats, the
immunoreactivity of CrA and SP decreased significantly but
was restored after testosterone supplementation. Although the
nature and functions of many of these substances in Leydig
cells remain unknown, these results are consistent with the
hypothesis that the expression of some neuroendocrine markers
is hormonally controlled.
lg of oestradiol or oestradiol plus testosterone
propionate (25 mg every 3 days) for 15 days. The animals
were sacrificed and testicles were dissected and processed by
routine histological protocols. FSH and LH serum levels were
determined by radioimmunoassay. The visualization of antigens
was achieved by the streptavidin-peroxidase immunohistochemical
method. Antibodies against chromogranin A
(CrA), S-100 protein (S-100), P substance (PS), synaptofisin
(SYN), neurofilament protein (NF), gliofibrillary acidic protein
(GFAP) and neuron specific enolase (NSE) were used. The
mean LH and FSH serum concentrations were consistently
suppressed with hormonal treatments. Intermediate filaments
(NF and GFAP) showed no difference in their expression. The
expression of S-100, NSE and SYN was significantly lower in
both hormone-treated groups. In oestradiol-treated rats, the
immunoreactivity of CrA and SP decreased significantly but
was restored after testosterone supplementation. Although the
nature and functions of many of these substances in Leydig
cells remain unknown, these results are consistent with the
hypothesis that the expression of some neuroendocrine markers
is hormonally controlled.