Application of natamycin and farnesol as bioprotection agents to inhibit biofilm formation of yeasts and foodborne bacterial pathogens in apple juice processing lines

AGUSTÍN, MARÍA DEL ROSARIO; TARIFA, MARÍA CLARA; VELA-GUROVIC, MARÍA SOLEDAD; BRUGNONI, LORENA INÉS

FOOD MICROBIOLOGY

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Año: 2022 vol. 109

0740-0020

Biofilms serve as a reservoir for pathogenic and spoilage microorganisms, and their removal from different surfaces is a recurring problem in the beverage industry. This study aimed to investigate the effect of a combination of natamycin (NAT, 0.01 mmol/l) and farnesol (FAR, 0.6 mmol/l) against biofilms on ultrafiltration (UF) membranes and stainless steel (SS) surfaces using apple juice as food matrix. The co-adhesion of Rhodotorula mucilaginosa, Candida tropicalis, C. krusei and C. kefyr (mixed yeast) with Listeria monocytogenes, Salmonella enterica or Escherichia coli O157:H7 (multi-species) in presence of NAT+FAR was evaluated for 2, 24, 48 h. In biofilms treated with NAT+FAR were observed by cell quantification and microscopy, inhibition of the filamentous yeast forms, disruption of the tridimensional structure and a high detachment of yeast cells. NAT+FAR affected the biofilms independently of the surfaces used and the presence (or not) of bacteria. L. monocytogenes was the most susceptible (p