Microfluidic Immunosensor with Micro Magnetic Beads coupled to Carbon-based Screen-Printed Electrodes (SPCEs) for determination of Botrytis cinerea in Tissue of Fruits

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: New York; Año: 2010 vol. 58 p. 11201 - 11206

0021-8561

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> A wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs and fundamentally fruits can be affected by grey mould caused by the fungal pathogen Botrytis cinerea. This paper describes the development of a microfluidic immunosensor with micro magnetic beads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for rapid and sensitive quantification of B. cinerea in apple (Red-delicious), table grape (pink Moscatel) and pear (William’s) tissues. The detection of B. cinerea was carried out using a competitive immunoassay method based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl- modified MMBs. The total assay time was 40 min and the calculated detection limit was 0.008 μg mL−1. Moreover the intra and inter-assay coefficients of variation were below 7%. The developed method allowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful for application in the agricultural industry.