Synthesis and performance of 3D-Megaporous structures for enzyme immobilization and protein capture

BIBI NS; GAVARA PR; SOTO ESPINOSA SL; GRASSELLI M; FERNÁNDEZ-LAHORE M

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2011 vol. 27 p. 1329 - 1338

8756-7938

The preparation of megaporous bodies, with potential applications in biotechnology, wasattempted by following several strategies. As a first step, naive and robust scaffolds were producedby polymerization of selected monomers in the presence of a highly soluble cross-linkeragent. Ion-exchange function was incorporated by particle embedding, direct chemical synthesis,or radiation-induced grafting. The total ionic capacity of such systems was 1.5 mmol Hþ/g, 1.4 mmol Hþ/g, and 17 mmol Hþ/g, respectively. These values were in agreement with theability to bind model proteins: observed dynamic binding capacity at 50% breakthrough was%7.2 mg bovine serum albumin/g, %7.4 hen egg-white lysozyme (HEWL) mg/g, and %108HEWL mg/g. In the later case, total (static) binding capacity reached 220 mg/g. It wasobserved that the structure and size of the megapores remained unaffected by the grafting procedurewhich, however, allowed for the highest protein binding capacity. Lysozyme supportedon grafted body showed extensive clarification activity against a Micrococcus lysodekticussuspension in the flow-through mode, i.e., 90% destruction of suspended microbial cells wasobtained with a residence time 18 min. Both protein capture and biocatalysis applicationsare conceivable with the 3D-megaporous materials described in this work