INVESTIGADORES
ROMERO eder Lilia
artículos
Título:
Curcumin-Loaded Lipid and Polymeric Nanocapsules Stabilized by Nonionic Surfactants: An In Vitro and In Vivo Antitumor Activity on B16F10 Melanoma and Macrophage Uptake Comparative Study
Autor/es:
LETÍCIA MAZZARINO, LUÍS F. C. SILVA, JULIANA C. CURTA, MARLEY A. LICÍNIO, ALINE COSTA, LETÍCIA K. PACHECO, JARBAS M. SIQUEIRA, JORGE MONTANARI, EDER ROMERO, JAMIL ASSREUY, MARIA C. SANTOS-SILVA, ELENARA LEMOS-SENNA
Revista:
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Editorial:
AMER SCIENTIFIC PUBLISHERS
Referencias:
Año: 2011 vol. 7 p. 1 - 9
ISSN:
1550-7033
Resumen:
Curcumin is a polyphenol obtained from the plant Curcuma longa (called turmeric) that displays
several pharmacological activities, including anti-inflammatory, antioxidant, antimicrobial and antitumoral
activity, but clinical use has been limited by its poor solubility in water and, consequently,
minimal systemic bioavailability. We have therefore formulated the drug into nanocarrier systems in
an attempt to improve its therapeutic properties. This study evaluates the effect of intraperitoneally
administered nanocapsules containing curcumin on subcutaneous melanoma in mice inoculated
with B16-F10 cells, and on the cytotoxicity activity against B16-F10 cells in vitro. Phagocytic uptake
of formulations was also evaluated upon incubation with macrophage J774 cells by fluorescence
microscopy. Lipid and polymeric nanocapsules were prepared by the phase inversion and nanoprecipitation
methods, respectively. The uptake of the lipid nanocapsules prepared using Solutol
HS15 was significantly reduced in J774 cells. Curcumin, as free drug or as drug-loaded nanocapsules,
was administrated at a dose of 6 mg/kg twice a week for 21 days. Free drug and curcuminloaded
nanocapsules significantly reduced tumor volume (P < 0.05 vs. control), but no difference
was found in the antitumor activity displayed by lipid and polymeric nanocapsules. This assumption
was supported by the in vitro study, in which free curcumin as well as loaded into nanocapsules
caused significant reduction of cell viability in a concentration- and time-dependent manner.in vitro. Phagocytic uptake
of formulations was also evaluated upon incubation with macrophage J774 cells by fluorescence
microscopy. Lipid and polymeric nanocapsules were prepared by the phase inversion and nanoprecipitation
methods, respectively. The uptake of the lipid nanocapsules prepared using Solutol
HS15 was significantly reduced in J774 cells. Curcumin, as free drug or as drug-loaded nanocapsules,
was administrated at a dose of 6 mg/kg twice a week for 21 days. Free drug and curcuminloaded
nanocapsules significantly reduced tumor volume (P < 0.05 vs. control), but no difference
was found in the antitumor activity displayed by lipid and polymeric nanocapsules. This assumption
was supported by the in vitro study, in which free curcumin as well as loaded into nanocapsules
caused significant reduction of cell viability in a concentration- and time-dependent manner.< 0.05 vs. control), but no difference
was found in the antitumor activity displayed by lipid and polymeric nanocapsules. This assumption
was supported by the in vitro study, in which free curcumin as well as loaded into nanocapsules
caused significant reduction of cell viability in a concentration- and time-dependent manner.in vitro study, in which free curcumin as well as loaded into nanocapsules
caused significant reduction of cell viability in a concentration- and time-dependent manner.