Pectin-based inks development for 3D bioprinting of scaffolds

PASSAMAI, VERÓNICA E.; ALVAREZ, VERA; RODENAK-KLADNIEW, BORIS; KATZ, SERGIO; CASTRO, GUILLERMO R.

JOURNAL OF POLYMER RESEARCH

SPRINGER

Lugar: Berlin; Año: 2022 vol. 30 p. 35 - 45

1022-9760

3D bioprinting allows creative ideas for 3D scaffold development. Therefore, it enhances the creation of customized dressingsfor tissue regeneration and the wound healing process. A relevant requirement when employing hydrogels in extrusion-basedbioprinting (EBB), is to maintain design fidelity and shape of printed structures. In this work, three novel biopolymeric inkswere formulated of which components are pectin (Pe) with the addition of carboxymethylcellulose (CMC) and microcrystallinecellulose (MCC). Specific methods for study extrudability, printability, physicochemical properties, and cytotoxicityof inks and 3D structures were proposed. 3D models of medium and high printing complexity were developed. Pe + MCCscaffold presents the best square interconnected channels (Printability≈ 1). Young?s modulus of Pe and Pe + MCC scaffoldsare in the same range of values as the skin modulus. Pe scaffold presents the highest water retention capacity. From thecytotoxicity test, the three inks showed well in vitro biocompatibility with L929 fibroblast cells. These results suggest thatPe and Pe + MCC biopolymer inks can potentially be implemented for developing 3D printed personalized dressings forwound healing treatment.