Kinetic fluorimetric determination of the antineoplastic methotrexate (MTX) in human serum

A. ESPINOSA-MANSILLA,* I.D. MERAS, A. Z. MADERA, M. L. PEDANO, C. FERREIRA.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Elsevier

Año: 2002 vol. 29 p. 851 - 858

0731-7085

A kinetic study of the oxidation of methotrexate (MTX) in acidic medium and in the presence of potassium permanganate has been made on the basis of the fluorescence?time curves. A kinetic method for the determination of MTX was developed with a range of application between 0.22 and 3.30 M. The proposed kinetic method permits us to determine MTX in human serum and to avoid the natural fluorescence of the serum. A detection limit of 0.18M. The proposed kinetic method permits us to determine MTX in human serum and to avoid the natural fluorescence of the serum. A detection limit of 0.18 M was calculated in the presence of ascorbic acid as activator. Only 100 s per sample is necessary for the analysis. The interference of pteridin derivatives and the rescue agent folinic acid (leucovorin) was tested.M was calculated in the presence of ascorbic acid as activator. Only 100 s per sample is necessary for the analysis. The interference of pteridin derivatives and the rescue agent folinic acid (leucovorin) was tested.