Effect of the hexanic extract of Achyrocline satureioides on Paenibacillus larvae growth, pathogen of Apis mellifera

M. F. PALETTI ROVEY; D. C. PIMENTEL BETANCURT; M.DE LA P. MOLINE; M. DE LAS M. OLIVA

Ab Intus

Facultad de Agronomía y Veterinaria - Universidad Nacional de Río Cuarto

Año: 2022 p. 33 - 49

2618-2734

The health of Apis mellifera hives is an important issue due to the ecologicaland economic role that bees play. Paenibacilluslarvae, a spore-forming bacillus, is the main bacterial pathogen of beeslarvae and the causative agent of American foulbrood. This disease causesconsiderable economic losses and its control is a challenge for beekeepersaround the world. Therefore, there is a need to find effective treatments andnatural products derived from medicinal plants are being studied. In thepresent work, the Minimum Inhibitory Concentration (MIC) and the Minimum BactericidalConcentration (MBC) of the hexanic extract of Achyrocline satureioides were determined, obtaining values ​​of0.30 μg/ml and 1.17 μg/ml, respectively. The growth parameters of P. larvae 9 (ERIC I) were determined,and then the effect of different concentrations of the hexanic extract(sub-inhibitory, inhibitory, sub-bactericidal and bactericidal) on thebacterial growth was evaluated. The hexanic extract showed a good inhibitoryeffect regardless of the concentrations tested. Bacterial growth wassignificantly affected at sub-bactericidal and bactericidal concentrations. Ourresults demonstrated the effectiveness of the hexanic extract of A. satureioides on P. larvae growth, constituting a promising alternative for Americanfoulbrood control.