Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

PICCINNI, FLORENCIA ELIZABETH; ONTAÑON, ORNELLA MAILÉN; GHIO, SILVINA; SAUKA, DIEGO HERMAN; TALIA, PAOLA MÓNICA; RIVAROLA, MÁXIMO LISANDRO; VALACCO, MARÍA PIA; CAMPOS, ELEONORA

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2018

1364-5072

ABSTRACTAimsLignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value-added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost-efficient enzymatic cocktails.Methods and ResultsWe identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboximethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exo-glucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo- and exo-glucanases, xylanases, a xyloglucanase, an arabinofuranosidase/ β-xylosidase, a β-glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10.ConclusionsGrowth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes.Significance and Impact of the StudyCellulomonas sp. B6 could serve as a source of lignocellulose degrading enzymes applicable to bioprocessing and biotechnological industries.