Copper-flavonoid family of complexes involved in alkaline phosphatase activation

GADDI, GISELA M.; CARO-RAMÍREZ, JANETSI Y.; PARENTE, JULIANA E.; WILLIAMS, PATRICIA A. M.; FERRER, EVELINA G.

BIOMETALS

SPRINGER

Año: 2023

0966-0844

The flavonoid naringenin and a family of naringenin derivative Cu(II) complexes having phenanthroline-based second ligands were selected to study alkaline phosphatase activation. This enzyme plays a critical role in tissue formation, increasing the inorganic phosphate formation, favoring mineralization, and being essential to producing bone mineralization. The effects of those compounds on the function and structure of the enzyme were evaluated by kinetic measurements, fluorescence, FTIR, and UV-Vis spectroscopies. The results showed that naringenin did not affect alkaline phosphatase activity, having a value of the Michaelis–Menten-constant close to the enzyme (Km = 3.07 × 10–6). The binary complex, Cu(II)-naringenin, and the ternary complex Cu(II)-naringenin-phenanthroline behaved as an enzyme activator in all the concentrations range used in this study. Those complexes increased in c.a. 1.9% the catalytic efficiency concerning enzyme and naringenin.