Construction of electroactive polyamine-enzyme assemblies nondependent on the electrical charge

CORIA-ORIUNDO, LUCY L.; CORTEZ, M. LORENA; HERRERA, SANTIAGO E.; AZZARONI, OMAR; BATTAGLINI, FERNANDO

SYNTHETIC METALS

ELSEVIER SCIENCE SA

Año: 2023 vol. 294

0379-6779

The assembly of polyelectrolytes and biomacromolecules (proteins, DNA) has attracted great attention in several fields related to medicine, molecular biology, and clinical chemistry. Here, a quick and easy multicomponent self-assembly is used to unite two species with the same charge to form an electroactive polyamine-enzyme assembly. Phosphate ions are added as a crosslinker agent to create a stable structure. Osmium-derivatized polyallylamine is combined with horseradish peroxidase (pI = 8.6) or PQQ-glucose dehydrogenase (pI = 9.5) on graphite electrodes. The assemblies are built by layer-by-layer or one-pot deposition. In both cases, the electrocatalytical signals observed are comparable to the state-of-the-art results. The proposed construction method represents a new alternative for the assembly of polyamines with proteins independently of their charge.