INVESTIGADORES
LESCANO LOPEZ Carlos Ignacio
artículos
Título:
Arabidopsis AZG2 Transports Cytokinins in vivo and Regulates Lateral Root Emergence
Autor/es:
TOMÁS TESSI; SABINE BRUMM; EVA WINKLBAUER; BENJAMIN SCHUMACHER; GEORGINA PETTINARI; IGNACIO LESCANO; CLAUDIO GONZÁLEZ; DIERK WANKE; VERÓNICA MAURINO; KLAUS HARTER; MARCELO DESIMONE
Revista:
NEW PHYTOLOGIST
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2020
ISSN:
0028-646X
Resumen:
Cytokinin and auxin are key regulators of plant growth and development. During thelast decade transport mechanisms turned out to be the key for the control of local andlong-distance hormone distributions. In contrast to auxin, cytokinin transport is poorlyunderstood. Here, we show that Arabidopsis thaliana AZG2, a member of the AZG purinetransporter family, acts as cytokinin transporter involved in root system architecturedetermination. Even though purines are substrates for both AZG1 and AZG2, we founddistinct transport mechanisms. The expression of AZG2 is restricted to a small group of cells surrounding the lateralroot (LR) primordia and induced by auxins. Compared to wild-type, mutants carryingloss-of-function alleles of AZG2 have higher LR density, suggesting AZG2 as beingpart of a regulatory pathway in LR emergence. Moreover, azg2 is partially insensitiveto exogenous cytokinin, which is consistent with the observation that the cytokininreporter TCSnpro:GFP showed lower fluorescence signal in the roots of azg2 comparedto wild-type. These results indicate a defective cytokinin signalling pathway in the region of LRprimordia. The integration of AZG2 subcellular localization and cytokinin transportcapacity data allowed us to propose a local cytokinin:auxin signalling model for theregulation of LR emergence.