INVESTIGADORES
RIVERA-POMAR Rolando
artículos
Título:
Neuropeptide precursor gene discovery in Rhodnius prolixus.
Autor/es:
ONS, SHEILA; STERKEL, M; DIAMBRA, L; URLAUB H,; RIVERA POMAR R
Revista:
INSECT MOLECULAR BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2011 vol. 20 p. 29 - 44
ISSN:
0962-1075
Resumen:
We show a straightforward workflow combining homology
search in Rhodnius prolixus genome sequence
with cloning by rapid amplification of cDNA ends and
mass spectrometry. We have identified 32 genes and
their transcripts that encode a number of neuropeptide
precursors leading to 194 putative peptides. We validated
by mass spectrometry 82 of those predicted neuropeptides
in the brain of R. prolixus to achieve the first
comprehensive genomic, transcriptomic and neuropeptidomic
analysis of an insect disease vector. Comparisons
of available insect neuropeptide sequences
revealed that the R. prolixus genome contains most of
the conserved neuropeptides in insects, many of them
displaying specific features at the sequence level.
Some gene families reported here are identified for the
first time in the order Hemiptera, a highly biodiverse
group of insects that includes many human, animal and
plant disease agents.