INVESTIGADORES
PERERA Maria Francisca
artículos
Título:
Genetic diversity among viruses associated with sugarcane mosaic disease in Tucumán, Argentina
Autor/es:
PERERA, M. F.; FILIPPONE, M. P.; RAMALLO, J.; CUENYA, M. I.; GARCÍA. M. L.; PLOPER, L. D. AND CASTAGNARO, A. P.
Revista:
PHYTOPATHOLOGY
Editorial:
The American Phytopathological Society
Referencias:
Año: 2009 vol. 99 p. 38 - 49
ISSN:
0031-949X
Resumen:
Sugarcane leaves with mosaic symptoms were collected in 200607 in
Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase
chain reaction (RT-PCR) restriction fragment length polymorphism
(RFLP) and sequencing of a fragment of the Sugarcane mosaic virusSugarcane mosaic virus
(SCMV) and Sorghum mosaic virus (SrMV) coat protein (CP) genes.
SCMV was detected in 96.6% of samples, with 41% showing the RFLP
profile consistent with strain E. The remaining samples produced eight
different profiles that did not match other known strains. SCMV distribution
seemed to be more related to sugarcane genotype than to geographical
origin, and sequence analyses of CP genes showed a greater
genetic diversity compared with other studies. SrMV was detected in
63.2% of samples and most of these were also infected by SCMV,
indicating that, unlike other countries and other Argentinean provinces,
where high levels of co-infection are infrequent, co-existence is common
in Tucumán. RFLP analysis showed the presence of SrMV strains M
(68%) and I (14%), while co-infection between M and H strains was
present in 18% of samples. Other SCMV subgroup members and the
SCMV was detected in 96.6% of samples, with 41% showing the RFLP
profile consistent with strain E. The remaining samples produced eight
different profiles that did not match other known strains. SCMV distribution
seemed to be more related to sugarcane genotype than to geographical
origin, and sequence analyses of CP genes showed a greater
genetic diversity compared with other studies. SrMV was detected in
63.2% of samples and most of these were also infected by SCMV,
indicating that, unlike other countries and other Argentinean provinces,
where high levels of co-infection are infrequent, co-existence is common
in Tucumán. RFLP analysis showed the presence of SrMV strains M
(68%) and I (14%), while co-infection between M and H strains was
present in 18% of samples. Other SCMV subgroup members and the
SCMV was detected in 96.6% of samples, with 41% showing the RFLP
profile consistent with strain E. The remaining samples produced eight
different profiles that did not match other known strains. SCMV distribution
seemed to be more related to sugarcane genotype than to geographical
origin, and sequence analyses of CP genes showed a greater
genetic diversity compared with other studies. SrMV was detected in
63.2% of samples and most of these were also infected by SCMV,
indicating that, unlike other countries and other Argentinean provinces,
where high levels of co-infection are infrequent, co-existence is common
in Tucumán. RFLP analysis showed the presence of SrMV strains M
(68%) and I (14%), while co-infection between M and H strains was
present in 18% of samples. Other SCMV subgroup members and the
SCMV was detected in 96.6% of samples, with 41% showing the RFLP
profile consistent with strain E. The remaining samples produced eight
different profiles that did not match other known strains. SCMV distribution
seemed to be more related to sugarcane genotype than to geographical
origin, and sequence analyses of CP genes showed a greater
genetic diversity compared with other studies. SrMV was detected in
63.2% of samples and most of these were also infected by SCMV,
indicating that, unlike other countries and other Argentinean provinces,
where high levels of co-infection are infrequent, co-existence is common
in Tucumán. RFLP analysis showed the presence of SrMV strains M
(68%) and I (14%), while co-infection between M and H strains was
present in 18% of samples. Other SCMV subgroup members and the
Sorghum mosaic virus (SrMV) coat protein (CP) genes.
SCMV was detected in 96.6% of samples, with 41% showing the RFLP
profile consistent with strain E. The remaining samples produced eight
different profiles that did not match other known strains. SCMV distribution
seemed to be more related to sugarcane genotype than to geographical
origin, and sequence analyses of CP genes showed a greater
genetic diversity compared with other studies. SrMV was detected in
63.2% of samples and most of these were also infected by SCMV,
indicating that, unlike other countries and other Argentinean provinces,
where high levels of co-infection are infrequent, co-existence is common
in Tucumán. RFLP analysis showed the presence of SrMV strains M
(68%) and I (14%), while co-infection between M and H strains was
present in 18% of samples. Other SCMV subgroup members and the
Sugarcane streak mosaic virus (SCSMV) were not detected. Our results
also showed that sequencing is currently the only reliable method to
assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may
not be sufficiently discriminating.
also showed that sequencing is currently the only reliable method to
assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may
not be sufficiently discriminating.
also showed that sequencing is currently the only reliable method to
assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may
not be sufficiently discriminating.
also showed that sequencing is currently the only reliable method to
assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may
not be sufficiently discriminating.
(SCSMV) were not detected. Our results
also showed that sequencing is currently the only reliable method to
assess SCMV and SrMV genetic diversity, because RT-PCR-RFLP may
not be sufficiently discriminating.