Core regulon of the global anaerobic regulator Anr targets central metabolism functions in Pseudomonas species

PAULA M. TRIBELLI; LUJAN ADELA; PARDO AGUSTIN; IBARRA JOSE G.; FERNANDEZ DO PORTO DARIO; SMANIA ANDREA; LOPEZ NANCY I

Scientific Reports

Springer Nature

Año: 2019

A comparative genome analysis of the global anaerobic regulator (Anr) regulon in five species ofPseudomonas with different life style was performed. Expression of this regulator was detected in allanalyzed Pseudomonas. The predicted Anr regulon (pan-regulon) consisted of 253 genes. However,only 11 Anr-boxes located upstream of qor/hemF, hemN, cioA/PA3931, azu, rpsL, gltP, orthologousto PA2867, cspD, tyrZ, slyD and oprG, were common to all species. Whole genome in silico predictionof metabolic pathways identified genes belonging to heme biosynthesis, cytochromes and EntnerDoudoroff pathway as members of Anr regulon in all strains. Extending genome analysis to 28Pseudomonas spp. spanning all phylogenetic groups showed Anr-boxes conservation in genes relatedto these functions. When present, genes related to anaerobic metabolism were predicted to holdAnr-boxes. Focused on the genomes of eight P. aeruginosa isolates of diverse origins, we observeda conserved regulon, sharing nearly 80% of the genes, indicating its key role in this opportunisticpathogen. The results suggest that the core Anr regulon comprises genes involved in central metabolismand aerobic electron transport chain, whereas those genes related to anaerobic metabolism and otherfunctions constitute the accessory Anr-regulon, thereby differentially contributing to the ecologicalfitness of each Pseudomonas species