Multilayered particle-packed column: Evaluation and comparison with monolithic and core?shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography

CHOCHOLOUS, PETR; GIL, RENATO; ACEBAL, CAROLINA C.; KUBALA, VICTOR; SATÍNSKÝ, DALIBOR; SOLICH, PETR

JOURNAL OF SEPARATION SCIENCE

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2017 vol. 40 p. 1225 - 1233

1615-9306

A recently presented new type of ?multilayered? organic?inorganic hybrid silica par-ticle packed columnYMC-Triart C18(50mm×4.6mm, 5μm)was used for the development of a sequential injection chromatographymethod for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orangeG, and para red) in selected food sea-sonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and comparedwith that of three types of columns typically used inmedium-pressure flowchromatography techniques (classicmonolithic, narrowmonolithic, and core?shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL+acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis