Simultaneous determination of flavor enhancers in stock cube samples by using spectrophotometric data and multivariate calibration

ACEBAL, CAROLINA C.; LISTA, ADRIANA G.; FERNANDEZ BAND, BEATRIZ S.

FOOD CHEMISTRY

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2008 vol. 106 p. 811 - 815

0308-8146

Spectrophotometric data followed by a suitable treatment of chemometric analysis were used for the simultaneous determination of monosodium glutamate (MSG), guanosine 50-monophosphate (GMP) and inosine 50-monophosphate (IMP) in stock cube samples, without any previous extraction step. By this way, the overlapping of the absorption spectra was resolved using a PLS-1 model. The concentration for experimental calibration matrix was varied between 5.03?34.2 µg mL-1 for IMP and GMP, and 448?1399 µg mL_1 for MSG. The relative errors of prediction (REPCV %) were 1.8, 2.8 and 3.1 for IMP, GMP and MSG, respectively. To verify the accuracy of the proposed method a recovery study on real samples was carried out with satisfactory results (92-110%).